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Email Newsletter

June 2013

Table of Contents

  1. Message from the President
  2. Cryo2013
  3. Upcoming Events
  4. Event Reports
  5. Cryobiology Journal

Do you have any news, reviews, articles, vacancies or training opportunities to share in the September newsletter?

If so please email them to [email protected]

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Click the links below to follow us and see our latest updates!



The editorial office would also welcome photographs  from CRYO2013 for the September newsletter so make sure you take your cameras to Bethesda in July and get snapping!

Message from The President

It’s been a busy time since the last newsletter, three months ago:

1.    Meetings

Amir Arav, Principal organiser of Controversies in Cryopreservation a) The 3rd annual meeting on “Controversies in Cryopreservation” was held March 21-23 in Berlin, Germany. A principal organizer of the meeting was Amir Arav, shown here on stage doing a remarkable demonstration of survival of red blood cells following freeze-drying with trehalose. 

This was a well-attended meeting, with about 300 participants, among whom were several members of the SFC as speakers. One aspect of the meeting well worth investigating for our own uses is that some sections of the meeting were designated as Continuing Medical Education (CME) courses. I suggest we investigate doing the same for   our own annual meeting, and we’ll be looking into that             Amir Arav, pictured above
possibility, with a discussion at the annual meeting of the
Board of Governors.

b) The 50th annual meeting of the SFC (July 28-31, Marriott Hotel, Bethesda, MD) is shaping up well. With the large number of biotech companies, independent research facilities, universities, and government research institutes in the Washington, DC, area, the Organizing Committee feels that the CRYO2013 annual meeting could be of interest to a large population of local and regional scientists and this could translate into a highly successful meeting. See http://www.societyforcryobiology.org/cryo-2013 for details. Note that the deadline for submitting abstracts has passed and that the program has been finalized.

Hilton Key Largo Resort

The Hilton Key Largo Resort, venue for the annual meeting of the American College of Cryosurgery 2014, above.

The annual meeting of the American College of Cryosurgery will be held January 15-19, 2014, in Key Largo, Florida. The SFC served as co-sponsors of the highly successful 2013 meeting, held aboard a cruise ship out of Miami in January, 2013, and we may participate again, a subject that will be discussed by the Board of Governors in Bethesda. 

Savannah, Georgia

d) The 2014 SFC meeting will be a joint meeting with the Society for In Vitro Biology (May 29-June 4, 2014, Hyatt Regency Hotel, Savannah, GA). Jason Acker, the Program Chair for the SFC worked tirelessly to arrange this meeting, for which we are grateful. Details of what is being planned will be made public as the time gets closer. The organizers are meeting this summer to finalize topics for the meeting. If you would like to make a suggestion, send it along to Jason ([email protected]).

The beautiful city of Savannah, right,

2.    Administrative Support

SfC New Editorial Assistanta) Support for the newsletter and website were taken over some months ago by David Rawson’s office, with Christie Roman providing the hands-on support, initially. She did a superb job, but she has now moved on to another position at the University of Bedfordshire. Her replacement is Nicole Evans, a New Zealander, now living in England.  Nicole will be attending the meeting in Bethesda. Be sure to introduce yourself; she is doing a fine job so far (she’s been on the job for a very short time), and she will be an increasingly central person in the SFC’s activities.

We welcome new Editorial Assistant, Nicole Evans, pictured left.

The contract with Portland for other administrative support will be terminated July 1, 2013, by mutual agreement. After some discussion among the Executive Committee, we approached David Rawson about taking on these additional duties, and it is working well. We have, for the first time in a long while, have the support functions collected in a single office. That will provide much needed stability and make possible day to day oversight that has been lacking with our former arrangements. We are grateful to David and the editorial staff for taking this on, particularly, Nicole Evans, who will be carrying a large fraction of the load.

c) The contract with Elsevier for publication of the journal has been under review for some months by everybody concerned. We are nearing the end of those discussions, with a very favorable outcome. Elsevier has been an excellent partner, and we look forward to a continued association with that company.

We’ll see you in Bethesda in July for a landmark meeting—our 50th!
John H. Crowe


Update on plans for the 50th annual meeting of the Society for Cryobiology, CRYO2013.

CRYO2013 promises to be a truly outstanding meeting on many levels. For the latest information please consult the CRYO2013 website, http://www.CRYO2013.org.

  • The hotel reservation link is open for you to take advantage of the discounted rates at the conference venue hotel.  Please note that the Bethesda North Marriot has confirmed to us that the discounted rate for conference delegates may not be available after June 29th so if you would like to take advantage of the special rate please book before this date.

  • The registration form is still available, if you have not yet registered, and should be returned to Dr Eric James, whose contact details are on the form.


The program has now been finalized and can be viewed at the following link:


International Young Cryobiology Researchers' Scavenger Hunt!

Smithsonian Museum of Natural History

Join the ICYR members on a scavenger hunt through the Smithsonian National Museum of Natural History!  Enjoy the company of your fellow Cryobiologists while discovering the secrets of the museum and tackling witty, tricky questions.

The event will take place from 2pm - 4pm Tuesday, July 30th, during the free afternoon of the conference.  It is open to both young and young at heart cryobiologists (i.e. there are no age restrictions!).                       
                                                                                  Inside the Smithsonian National Museum of Natural History, pictured right.

On a 90 minute expedition through the museum, you and your team members will hunt for dinosaurs, stuffed tigers and giraffes, a camping vampire, a flossing bison, live insects, the Hope Diamond and other jewels,  mummies, charred-bone from a         
real murder mystery and much more. 

On a whirlwind tour, you’ll travel to China, Africa, South America, the Ice Age, ancient oceans, and beyond.  The questions are designed so that you can find the answer only when you are in the right spot and looking and the right thing (Sorry, Googlers!).  You don’t need any previous knowledge to triumph—you just need good teamwork and comfortable shoes!

Admittance to the scavenger hunt is $20, which will be collected at the Annual Meeting. Transportation will be provided to and from the event.  

Please RSVP by July 12th to Allyson Fry Davidson at [email protected].

Upcoming Events

ASME Header

Mini-Symposium - Nano-Thermal Medicine - Bench Top to Bed Side: Challenges and Opportunities
14th - 19th July 2013, Minneapolis, USA

A mini-symposium on "Nano-Thermal Medicine - Bench Top to Bed Side: Challenges and Opportunities" is being organized as part of the 75th anniversary celebrations of the Heat Transfer Division at the 2013 ASME Summer Heat Transfer Conference, in Minneapolis, MN.  
This mini-symposium is being organized by Dr. Sankha Bhowmick (University of Massachusetts), Dr. Bumsoo Han (Purdue) and Dr. Ram Devireddy (Louisiana State University) and will include invited lectures/presentations from researchers specializing in the following areas:

  1. Nanoscale Heat Transfer (Measurement, Models and Applications) or Heat Transfer around Nanostructure in Biological Settings
  2. Quantitative Imaging and Diagnosis using Nanoparticles
  3. NanoMaterials in Bioengineering (Choice, Selection, Fabrication and Use  thereof) or Synthesis of Functional Nanostructures
  4. Delivery of NanoBioMaterials (Selective Targeting, "turn-on" and "turn-off" switches) or Mass Transport of Nanoparticles for Diagnosis and Drug Delivery
  5. Current Clinical Techniques in Nanothermal Medicine (Advantages and Limitations)
  6. Clinical/FDA/Translational Challenges (Regulatory Challenges)

Rationale:  Recent developments of nanotechnology enable a wide variety of nanostructures, whose properties can be tailored for diagnosis, imaging and treatment of diseases.  These nanostructures include liposomes, polymer micelles, dendrimers, drug nanocrystals, quantum dots, magnetic nanoparticles, gold nanoparticles/nanoshells, nanorods, nanotubes, and drug-polymer conjugates (collectively referred to as NPs here).  However, these structures are primarily characterized for their static properties including size, surface charge, thickness and hydrophobicity of surface coatings, and conjugation properties.  Thus, their in vivo behaviors, which are critically affected by complex interactions with the surrounding cells, fluid and extracellular matrix, are not well understood.  Xenograft models have been essential platforms to characterize the in vivo behavior of the NPs.  However, xenograft models only provide the net result of complex processes in vivo, making it difficult to establish mechanistic understanding of the NPs' behavior in vivo.  In order to properly design effective nano-therapuetics, thus, new characterization methods are highly desired which can predict the in vivo heat and mass transport processes around the NPs in biological settings.

IIR Workshop on Cryoprocessing of Biopharmaceuticals and Biomaterials
4th - 5th September 2013, Dresden Germany

The International Institute of Refrigeration Workshop on Cryoprocessing of Biopharmaceuticals and Biomaterials, is to be held 4th and 5th September 2013 in Dresden, Germany.

This workshop aims to provide an interdisciplinary scientific platform for a dialog and exchange of experiences between R&D-institutes and industry. The ILK Dresden and the Vienna University of Technology as the initiators and organizers of the workshop would like to invite all of you who have interest in that field to come to Dresden, Germany.

For more information please visit http://www.ilkdresden.de/index.php?id=1156

Society for Low Temperature Biology (SLTB) 2013 Annual General Meeting and Scientific Conference
6th - 9th October 2013, Hannover, Germany

The Society for Low Temperature Biology (SLTB) 2013 Annual General Meeting and Scientific Conference will take place 6th – 9th October in Hannover Germany, organised and hosted by The Institute of Multiphase Processes at Leibniz University, Hannover.

The SLTB was founded in 1964 with the purpose of promoting research into the effects of low temperatures on all types of organisms and their constituent cells, tissues and organs.   
The Society holds at least one main meeting each year. The programme will include both a symposium on a topical subject and a session of free communications on any aspect of low temperature biology. Recent symposia have included long-term stability, preservation of aquatic organisms, cryopreservation of embryos and gametes, preservation of plants, low temperature microscopy, vitrification, freeze drying and tissue banking.

For more information please visit http://www.sltb.info


ESBB Annual Conference
8th - 11th October 2013, Verona, Italy

The ESBB, was founded in August 2010 as a regional chapter of The International Society for Biological and Environmental Respositories (ISBER).  This year marks ESBB’s third annual conference.

The theme of this year’s conference is “Biobanking for the Future” and will offer a wide variety of interesting content from across the biobanking spectrum. Sessions include: Global Biobanking, Ancient DNA, Biobanking for Personalised Medicine, Biobanking for Conservation, Sustainability of Biobanks and the Financial Crisis, and Innovative Biobanking.

Exciting new features this year include the interactive sessions in which audience participation will be facilitated by an electronic voting system. Topics for these interactive sessions include: “The Life in Data” and “Everything Your Wanted to Know About Collaborating With Industry … But Were Afraid To Ask” and “Harmony for Conservation”. This year also sees the first training course entitled “The Pathology of Biobanking” which will be presented by ESBB and the local organizers.

1st July – Closing date for abstracts and early registration.

For more information please visit http://www.esbb.org/meetings.html

Event Reports

Cold in Biology Logo

May 20th – 21st 2013, Kharkov, Ukraine

The Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences in Ukraine together with the UNESCO Chair in Cryobiology organize the annual Conference Cold in Biology and Medicine: Current problems in cryobiology, transplantology, and biotechnology.  The aim of the conference is to encourage undergraduate and postgraduate students to share their research and give an overview  of the activities of today’s young scientists to the academic community.

The conference is devoted to the investigation of various aspects of low temperature effects on biologicals: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing, cell and tissue culturing etc.; response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation; developing of cryobiological and cryomedical devices; and organisation and functioning of low temperature banks.

This year there were 26 presentations and international participation from UNESCO Chair Professors Colin Green and Barry Fuller (UK).  All the attendees took part in the competition for the best presentation and the jury chose the following four winners:

Vitaliy Mutsenko (pictured, right): The study of cultivation and Vitaliy Mutsenko, Prizewinner
differentiation of mesenchymal stromal cells within the scaffolds
based on skeletons derived from the marine sponge
Olga Mykhaylova: Apoptosis stages in cord blood nucleated cells depending on cryopreservation method

Elena Rogulskaya: Investigation of cryopreservation of human mesenchymal stromal cells using oligosaccharides

Anna Trofimova: Hypothermic experiments in rats: electrocardiophysiological indices when cooling head and neck.

Prize Winner, Vitaliy Mutsenko, pictured right.

Thank-you to Vitaliy Mutsenko for this event report.   

ACCryo2013ACC Logo
2nd - 7th January 2013, Florida and Mexico

Between January 2nd - 7th the American College of Cryosurgery held its
16th annual meeting (ACCryo2013). The meeting was co-sponsored by
the Society for Cryobiology and endorsed by the International Institute of Refrigeration and the Cryogenic Society of America.  Over one hundred participants representing eleven countries attended.

The venue was unique in that there was an opening land-based session in Miami, Florida followed by a four days of sessions aboard a cruise ship with ports of call at Key West, Florida and Cozumel, Mexico.

Technical sessions focused on both biopreservation and cryoablation with a strong emphasis on the linkages between the principles of these divergent fields.  Six of the eight scientific sessions were organized and chaired by individuals holding dual membership in the Society for Cryobiology and the ACC. 

Plenary lectures we presented by Dr. Truls E. Bjerkland Johansen (Cryosurgical ablation of the prostate)(Norway), Dr. Kenneth B. Storey (Mammalian hibernation - clinical implications)(Canada), Dr. Michael Sabel (Cryo-immunology)(USA), Dr. John G. Baust (Advances in cryoablation)(USA) and Dr. Jim Vaught (NCI cancer specimen biorepositories)(USA).
In addition Dr. John H. Crowe, President of the Society for Cryobiology, provided the President's Welcome.

Next year's ACCryo2014 will be held between January 15th - 19th at the Hilton Key Largo Resort in Florida (http://www.cryoconference.com). This meeting will provide a balance between molecular approaches to understanding cellular responses to low temperature and clinical outcomes following translation of these fundamental principles for disease control.

Members of the Society for Cryobiology are cordially invited to join with the ACC to advance our knowledge in areas of mutual interest.

For more information about the ACC please visit http://www.accryosurgery.org

Thank-you to John G. Baust for this event report

Cryobiology Journal

Vol. 66., issue 3, pp201 - 364 (June 2013)

Review Article

  • Cryopreservation of articular cartilage
    Alireza Abazari, Nadr M. Jomha, Janet A.W. Elliott, Locksley E. McGann

Regular Articles

  • Wound dressing material containing lyophilized allogeneic cultured cells
  • Kazutaka Soejima, Katsumi Shimoda, Tsutomu Kashimura, Takashi Yamaki, Taro Kono, Hiroyuki Sakurai, Hiroaki Nakazawa

  • Cryopreservation of alginate encapsulated mesenchymal stromal cells
    Alexey I. Pravdyuk, Yuri A. Petrenko, Barry J. Fuller, Alexander Y. Petrenko

  • An improved freeze-dried PRP-coated biodegradable material suitable for connective tissue regenerative therapy
    Makoto Horimizu, Tomoyuki Kawase, Yu Nakajima, Kazuhiro Okuda, Masaki Nagata, Larry F. Wolff, Hiromasa Yoshie

  • Viability of human ovarian tissue confirmed 5 years after freezing with spontaneous ice-formation by autografting and chorio-allantoic membrane culture
    Vladimir Isachenko, Ingrid Orth, Evgenia Isachenko, Peter Mallmann, Doris Peters, Torsten Schmidt, Bernd Morgenstern, Dolores Foth, Bettina Hanstein, Gohar Rahimi

  • Investigating the cryoablative efficacy of a hybrid cryoprobe operating under freeze–thaw cycles
    X. Zhao, K.J. Chua

  • Effects of post-exercise recovery in a cold environment on muscle glycogen, PGC-1α, and downstream transcription factors
    Dustin Slivka, Matthew Heesch, Charles Dumke, John Cuddy, Walter Hailes, Brent Ruby

  • Cryopreservation of human embryonic stem cells by a programmed freezer with an oscillating magnetic field
    Pei-Yi Lin, Yao-Chen Yang, Shih-Han Hung, Sheng-Yang Lee, Maw-Sheng Lee, I-Ming Chu, Shiaw-Min Hwang

  • Cytokeratin distribution and expression during the maturation of mouse germinal vesicle oocytes after vitrification
    Xia Wei, Fu Xiangwei, Zhou Guangbin, Xu Jing, Wang Liang, Du Ming, Yuan Dianshuai, Yue Mingxing, Tian Jianhui, Zhu Shien

  • Effects of hibernation on regulation of mammalian protein phosphatase type-2-A
    Cheng-Wei Wu, Anthony J. Reardon, Kenneth B. Storey

  • Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender
    Abozar Najafi, Mahdi Zhandi, Armin Towhidi, Mohsen Sharafi, Abbas Akbari Sharif, Mahdi Khodaei Motlagh, Felipe Martinez-Pastor

  • Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries
    Yutaka Banno, Kiyomi Nagasaki, Marino Tsukada, Yuko Minohara, Junko Banno, Kazuhiro Nishikawa, Kazunori Yamamoto, Kei Tamura, Tsuguru Fujii

  • Cryobiology of cephalopod (Illex coindetii) spermatophores
    Vanesa Robles, Felipe Martínez-Pastor, Giuliano Petroni, Marta F. Riesco, Anna Bozzano, Roger Villanueva

  • Thermal and hemodynamic response to whole-body cryostimulation in healthy subjects
    Pawel Zalewski, Jacek J. Klawe, Joanna Pawlak, Malgorzata Tafil-Klawe, Julia Newton

  • An experimental study on periodontal regeneration after subcutaneous transplantation of rat molar with and without cryopreservation: An in vivo study
    Sarah Staels, Peter De Coster, Anne Vral, Liesbeth Temmerman, Guy De Pauw

  • An efficient and mass reproducible method for vitrifying mouse embryos on a paper in cryotubes
    Kun-Hsiung Lee, Jung-Ching Sun, Chin-kai Chuang, Shyh-Forng Guo, Ching-Fu Tu, Jyh-Cherng Ju

  • Forced-convective vitrification with liquid cryogens
    Shaw-Ruey Lyu, Jen-Hung Huang, Wei-Hung Shih, Yung-Jiun Chen, Wen-Hsin Hsieh

  • Osteogenesis of cryopreserved osteogenic matrix cell sheets
    Takamasa Shimizu, Manabu Akahane, Tomoyuki Ueha, Akira Kido, Shohei Omokawa, Yasunori Kobata, Keiichi Murata, Kenji Kawate, Yasuhito Tanaka

  • Effect of two sulfur-containing amino acids, taurine and hypotaurine in European sea bass (Dicentrarchus labrax) sperm cryopreservation
    S. Martínez-Páramo, P. Diogo, M.T. Dinis, F. Soares, C. Sarasquete, E. Cabrita

Brief Communications

  • Effect of buffer systems and disaccharides concentration on Podoviridae coliphage stability during freeze drying and storage
    C. Dini, P.J. de Urraza


  • Abstracts of papers, presentations and posters presented at ACCryo 2013, held in Miami, USA January 2nd - 7th 2013.