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September 2016

Table of Contents

  1. Message from the President
  2. New Members
  3. Notes from the Director's Desk
  4. CRYO2016
  5. Cryobiology Journal
  6. 2016 Officers and Governors
  7. 2017 Annual Meeting in China
  8. Call for Nominations for BoG
  9. Membership Renewal


New Members

We had a total of 17 new members join the Society from 5 countries since our last quarterly newsletter. A very warm welcome to all our new members!

Melnie Chow-shi-yee
Mathiew Nadeau
Tracey Turner
Praveen Saxena

Tian Zhou
Lili Zou
II Jeoung Yu
Chunsheng Jia
Wenyu Sun

Magda Teixeira

Maryam Hezavehei

Huiping Yang
Nilay Chakraborty
Brian Hawkins

Joseph Abram
Jedediah Lewis
Liga Wuri


Cryobiology Journal

October 2016, Volume 73 (ii)


 Regular Articles

Tissue-specific response of carbohydrate-responsive element binding protein (ChREBP) to mammalian hibernation in 13-lined ground squirrels
Samantha M. Logan, Kenneth B. Storey

Inhibition of skeletal muscle atrophy during torpor in ground squirrels occurs through downregulation of MyoG and inactivation of Foxo4
Yichi Zhang, Shannon N. Tessier, Kenneth B. Storey

Vitrification transiently alters Oct-4, Bcl2 and P53 expression in mouse morulae but does not affect embryo development in vitro
Guangbin Zhou, Yan Zeng, Jiang Guo, Qinggang Meng, Qingyong Meng, Gongxue Jia, Keren Cheng, Changjun Zeng, Ming Zhang, Guoshi Liu, Shi'en Zhu

Mechanisms of islet damage mediated by pancreas cold ischemia/rewarming
Keiko Omori, Eiji Kobayashi, Jeffrey Rawson, Masafumi Takahashi, Yoko Mullen

Supplementation freeze-thawed media with selenium protect adipose-derived mesenchymal stem cells from freeze-thawed induced injury
Arash Valadbeygi, Tahere Naji, Afshin pirnia, Mohammadreza Gholami

Influence of Aeromonas hydrophila and Pseudomonas fluorescens on motility, viability and morphometry of cryostored silver barb (Barbodes gonionotus) sperm
Traimat Boonthai, Weerasith Khaopong, Jumlong Sangsong, Verapong Vuthiphandchai, Subuntith Nimrat

A root bond between ice and antifreeze protein
Timothy C. Hawes

Neural differentiation potential of sympathoadrenal progenitors derived from fresh and cryopreserved neonatal porcine adrenal glands
G.A. Bozhok, O.S. Sidorenko, E.M. Plaksina, T.M. Gurina, A.N. Sukach, V.S. Kholodnyy, V.D. Ustichenko, S.B. Bilyavskaya, T.P. Bondarenko, E.I. Legach

Cryopreservation of human spermatozoa with minimal non-permeable cryoprotectant
Jie Liu, Cigdem Tanrikut, Diane L. Wright, Gloria Y. Lee, Mehmet Toner, John D. Biggers, Thomas L. Toth

Vitrification at Day3 stage appears not to affect the methylation status of H19/IGF2 differentially methylated region of in vitro produced human blastocysts
Marzieh Derakhshan-Horeh, Farid Abolhassani, Farnoosh Jafarpour, Ashraf Moini, Khadijeh Karbalaie, Sayyed Morteza Hosseini, Mohammad Hossein Nasr-Esfahani

Increased cryosurvival of osteosarcoma cells using an amphipathic pH-responsive polymer for trehalose uptake
S.A. Mercado, N.K.H. Slater

Intracellular ice formation in mouse zygotes and early morulae vs. cooling rate and temperature-experimental vs. theory
Bo Jin, Shinsuke Seki, Estefania Paredes, Juan Qiu, Yanbin Shi, Zhenqiang Zhang, Chao Ma, Shuyan Jiang, Jiaqi Li, Feng Yuan, Shu Wang, Xiaoguang Shao, Peter Mazur

The preservation of vital functions in cat ovarian tissues during vitrification depends more on the temperature of the cryoprotectant exposure than on the sucrose supplementation
Lara Mouttham, Pierre Comizzoli

Thermal conductivity of the cryoprotective cocktail DP6 in cryogenic temperatures, in the presence and absence of synthetic ice modulators
Lili E. Ehrlich, Jonathan A. Malen, Yoed Rabin

Correlation between the cryosurvival, cell number and diameter in bovine in vitro produced embryos
Alper Kocyigit, Mesut Cevik

Influence of cooling and thawing conditions and cryoprotectant concentration on frozen-thawed survival of white-naped crane (Antigone vipio) spermatozoa
Saritvich Panyaboriban, Budhan Pukazhenthi, Megan E. Brown, Chris Crowe, Warren Lynch, Ram P. Singh, Mongkol Techakumphu, Nucharin Songsasen

In vitro fertilization with cryopreserved spermatozoa in small pieces of gonad of the scallop Argopecten purpuratus (Lamarck, 1819)
Enrique Dupré, Alejandra Covarrubias, Merari Goldstein, Alicia Guerrero, Herman Rojas

Strong tolerance to freezing is a major survival strategy in insects inhabiting central Yakutia (Sakha Republic, Russia), the coldest region on earth
N.G. Li

Dimethyl sulfoxide attenuates nitric oxide generation via modulation of cationic amino acid transporter-1 in human umbilical vein endothelial cells
Ohad S. Bentur, Tamara Chernichovski, Merav Ingbir, Talia Weinstein, Idit F. Schwartz

Open encapsulation-vitrification for cryopreservation of algae
Namrata Kumari, Mukesh Kumar Gupta, Raghubansh Kumar Singh

Cryopreservation of lipid bilayers by LEA proteins from Artemia franciscana and trehalose
Daniel S. Moore, Steven C. Hand

Potassium ions in extender differentially influence the post-thaw sperm motility of salmonid fish
Sylwia Judycka, Joanna Nynca, Ewa Liszewska, Stefan Dobosz, Tomasz Zalewski, Andrzej Ciereszko

The feasibility and safety of cryoablation as an adjuvant therapy with transurethral resection of bladder tumor: A pilot study
Shenghua Liu, Limin Zhang, Lujia Zou, Hui Wen, Qiang Ding, Haowen Jiang

Polarized light scanning cryomacroscopy, part I: Experimental apparatus and observations of vitrification, crystallization, and photoelasticity effects
Justin S.G. Feig, David P. Eisenberg, Yoed Rabin

Polarized light scanning cryomacroscopy, part II: Thermal modeling and analysis of experimental observations
Justin S.G. Feig, Prem K. Solanki, David P. Eisenberg, Yoed Rabin

Brief Communications

Lipid composition in common carp (Cyprinus carpio) sperm possessing different cryoresistance
Y. Horokhovatskyi, S. Sampels, J. Cosson, O. Linhart, M. Rodina, P. Fedorov, M. Blecha, B. Dzyuba

Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids
Seungki Lee, Yoshiko Iwasaki, Goro Yoshizaki

Comparative analysis of cryopreservation methods in Chlamydomonas reinhardtii
Chasity Scarbrough, Maureen Wirschell

 2016 Officers and Governors


President-Elect Dayong Gao
Past-President Erik Woods
Secretary Yuksel Agca
Treasurer Adam Higgins


Serean Adams
W. John Armitage
Robert Ben
James D. Benson
Gloria D. Elliott
Jens O. M. Karlsson
Barbara M. Reed

Willem Wolkers
Gang Zhao

 Do you have any news, reviews, articles, vacancies or training opportunities to share in the Summer newsletter?

If so, please email them to the editorial team at [email protected]

Membership Renewal 

If you haven't already renewed yet for 2016 you can still do it! Accounts that have not been renewed by the end of March have lapsed.  


Individual membership is available for the low price of $75. Student and retired members continue to benefit from free membership. 

An electronic subscription to our journal is included with your membership, but an additional supplement is payable by all members for a print subscription to Cryobiology.

To renew, visit our membership renewal page.


Find and connect with your Society for Cryobiology on any of the social media sites below!         








Message from the President

Where do we go from here?  Over the past year, the Executive Committee and the Board of Governors have been focusing our attention on addressing the immediate operational needs of the Society. Our one-year plan highlighted the need for us to focus on member recruitment and retention, annual meeting stabilization, bylaw and procedure review, internal staffing and member communications.  By focusing on these foundational activities, we are now in a very good position to be able to have the very important discussions around where we as a Society see ourselves in 5-10 years and what activities and actions we need to take to get us there.

As we undertake a review of our 3-5 year strategic plan and look outward 5-10 years, it is very important that we hear from all members of the Society.  At the CRYO2016 meeting in Ottawa, I had the opportunity to talk with many of you. You shared with me your excitement for the science, your desire to see us preserve our past, and your thoughts for how we can and need to take a stronger leadership role in the applied areas of cryopreservation and cryobiology.  It was great to hear your excitement, but now is the time to turn that excitement into action.  Over the next 3-6 months, the Board of Governors will be soliciting your feedback and will be inviting members to participate in small working groups to help us develop and frame our strategic plan.  I would encourage you to get involved and let us know what you think are the important areas that the Society for Cryobiology needs to focus on over the next 5-10 years.

As we get closer to the end of the calendar year, we will once again be holding our annual election of Governors. We have three positions opening up on the Board and the Nomination Committee has identified a slate of candidates. However, if you are interested in putting your name forward, we are still accepting nominations from the general membership. Please contact our Executive Director or Secretary if you have any questions about the nomination by petition process. Deadline for nominations is October 16, 2016.

For those that are interested in getting more involved with the Society but are not inclined to stand for election, we need you too! Please consider getting involved with one of the many Board committees or working groups. The time commitment is not onerous and it provides an excellent opportunity to network and engage with our international membership.  Please contact me at [email protected] if you are interested in getting involved.

I would like to take this opportunity to thank Gowri Dep who has been working quietly behind the scenes in our Editorial Office to support our Editor-in-Chief, manage our member communications (including this newsletter), process member applications and renewals and assisting with our annual meeting planning.  Gowri will be leaving us in November when Nicole Evans returns from her maternity leave.  Thank you Gowri for your service to the Society!

Where do we go from here? Let’s answer this question together.


Notes from the Director's Desk

The 53rd Annual Meeting of the Society for Cryobiology, CRYO2016 in Ottawa, Canada on July 24 – 27, 2016 was a tremendous success from several points of view. Statistically there were 154 attendees from 20 countries including Australia, Brazil, Canada, Chile, China, Czech Republic, Finland, France, Germany, Hungary, India, Israel, Italy, Japan, South Korea, Russian Federation, Spain, Ukraine, United Kingdom, and the United States. There were 70 students or post-docs representing approximately 50% of the total attendees and the largest number of students at a recent meeting. The meeting consisted of 4 plenary sessions, 5 symposia, 20 sessions, 3 invited speakers, and a tribute to Dr Peter Mazur. Over 118 presentations with 72 accepted abstracts and 55 posters (not all exhibited) were presented from the original 180 submissions. This was also the first year that the Society took on the responsibility of organizing the annual meeting and not just the local committee. This required a coordinated effort involving the Executive Director (logistics), President (chair and programming), Treasurer (finance), local board member Rob Ben (programming and local onsite), SfC staff support (minutes, photos), and several committees (programming, finance, etc.). This year we began to go green and electronic with the use of Whova to display the meeting agenda, attendee list with photos and contact information, logistical information, meeting photographs, and the book of abstracts directly on your electronic devices.

If you were not able to attend this year’s conference, then you will want to plan ahead for next year’s 54th Annual Meeting in China. Next year’s joint meeting with several Chinese Societies including the Chinese Cryobiology Society, Chinese Blood Transfusion Association, Chinese Primate Center, and Chinese Biomedical Engineering Society will be held in Hefei, China on July 20 – 24, 2017. Although the Scientific Program has not been fully developed, the theme for the conference is Cryopreservation and Biobanking for Advanced Research and Modern Agriculture and P4 Precision Medicine.

The conference will be held at the gorgeous Hefei International Conventional Center and Hotel. Accommodations will be at the 5-Star Hefei International Convention Center Hotel where room rates are far less than $100USD/night and include breakfast, room service, Wi-Fi, the fitness center, and many other amenities. A full social program is being planned and includes museums, sea world, ancient city tours, parks, and regional mountain tours. Post-conference tours of nearby Beijing, Shanghai, and the stunning Yellow Mountains are also being considered to take advantage of this unique opportunity and location, so plan now to bring the entire family or that significant other in your life and enjoy beautiful China!

Over the past several months I have been working with Dr. Jens Karlsson facilitating the Board revision of the Society’s bylaws to make them more reflective of how the Society operates. All changes must also align with a number of changes in legislation and law in the state of Maryland, where the Society is a registered as a 501(C)(3) non-profit. Once the Board tentatively finalizes and approves the revised bylaws they will be sent to all members in good standing to review and respond within a 30-day comment period. The Board will then consider all the comments to the bylaws and make possible revisions. The final revised bylaws will then be sent back to the members to be voted on before the next annual meeting in China.

I presented the 2016-2020 Strategic Plan to the Board that they directed that I draft along with a complete explanation of how it was developed from input based on hour-long interviews with 23 of the Society’s most influential leaders, past and present, and informal interviews lasting about 30 minutes with 3 present members, 2 potential members, and several student members. This plan focusses on the key areas of: cryobiology and the SfC, governance structure and operation, annual meetings, the Cryobiology journal, membership, communication, and education. I also presented the 2016 Business and Tactical Plan that was developed in April 2016 with SfC staff input and has 48 specific actions with deadlines in addition to the 5 strategic directions, 18 specific goals, and 36 tactics. This is an action plan for work to be accomplished this year. The Board was very pleased with the documents and after some discussion agreed that these plans should be put on the SfC website for all members to see SfC’s path forward. I strongly recommend that all of you visit the SfC website and review the 5-year strategic plan and 1-year business plan.

I have now been in my role as Executive Director of the Society for 18 months and much has been accomplished during that time frame. However much more needs to be done to continue to improve the Society and make it relevant and important in today’s world. I look forward to continuing to get to know all of you and work with you to revitalize our Society.
Ed Kordoski

Cryo2016CRYO2016 was held at the Fairmont Château Laurier, Ottawa, Canada on 24-27 July. This year's conference saw the delivery of 162 presentations in total, with 121 oral and 41 poster presentations.

Highlights of the 53rd Annual Meeting of the Society for Cryobiology:

1. A tribute to Dr. Peter Mazur was given by John G Baust, Jason Acker and Adam Higgins.

2. John Bischof and Greg Fahy were presented with the Luyet Medal as the recent Society for Cryobiology Fellows.

3. International Cryobiology Young Researchers (ICYR) Events and Diefenbunker Tour

CRYO2016 provided young researches ample opportunity to network with fellow cryobiologists. A haunted tour of central Ottawa around the Fairmont Chateau Laurier was organised by Peter Kilbride of ICYR as well as a meal and a games night  in the historic Byward Market near the Fairmont. Attendees also had a chance to visit the Diefenbunker, which was built to house the Canadian leaders during a nuclear attach and now serving as a cold war museum. The spectacular nightly Northern Lights show on Parliament Hill was great for entertainment and socialising. 

Ghost Tour, Games Night & Diefenbunker Tour     

 Northern Lights - Parliament Hill                            


4Tiantian Zhang, Jens Karlsson and Barbara Reed were presented with the Service Awards in recognition of service as a Governor-at-Large for the Society for Cryobiology. Barry Fuller was recognized for his service as President-Elect, President, and Past-President of the Society. Our warmest congratulations to them all and thank you for your continued support!


5. Presentation of CRYO2016 Student Awards.

Students were recognised and rewarded for their excellent research in the field of Cryobiology. Miao Zhang (Germany) was the CRYO2016 Crystal Award winner, a prestigious award given for the best oral presentation. This year's Best Poster Award and Critser Travel Award was won by Vannesa Musca (Canada) and Jessica Poisson (Canada) respectively.


Student Travel Awards were presented to Kenneth Baumann (USA), Guan-qun Chen (China), Jaanika Edisi (Finland), Haritz Gurruchanga (Spain), Lothar Lauterbock (Germany), Sergio Mercado (UK), Vitali Mutsenko, JiaJi Pan (USA), Krishna Ramajayam (India), Iryna Trutiaeva (Ukraine) and Yuanyuan Zheng (China).

Congratulations to all the CRYO2016 award winners!!

5. Gala Banquet 

After a wonderful dinner and an evening of dance, 53rd Annual Meeting of the Society of Cryobiology drew to a close.

Click for more pictures of the CRYO2016 conference.

2017 Annual Meeting to be Held in China!

The Board of Governors is happy to announce that next year’s joint meeting with the Chinese Cryobiology Society, Chinese Blood Transfusion Association, Chinese Primate Center, and Chinese Biomedical Engineering Society will be held in Hefei, China on July 20 – 24, 2017.

Conference venue will be the 5-Star Hefei International Convention Center Hotel where room rates are less than $100USD/night and include breakfast, room service, Wi-Fi, the fitness center, and many other amenities.

The theme for the conference is Cryopreservation and Biobanking for Advanced Research and Modern Agriculture and P4 Precision Medicine. Watch for more information in the next coming months.


Call for Nominations for the Board of Governors

The Nomination Committee is calling for nominations for three Governor positions, which will be vacant at the end of the year. Current members of the Society for Cryobiology can nominate another member or self-nominate if they have 10 signatures (emails) from members in good standing order.  This documentation can then be sent to the secretary, Yuksel Agca at [email protected] who will be preparing the ballot documents for the election. If you would like to be actively involved and make a difference to the Society for Cryobiology, consider standing for election this year for the class of 2019 and serve on the Board of Governors. Currently James Benson, Ido Braslavsky, Ali Eroglu, Allison Huble, and Tiantian Zhang have agreed to run for the Class of 2019. Please note that nominations must be received by the end of the day on October 16, 2016.